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pduo plasmids  (InvivoGen)


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    Structured Review

    InvivoGen pduo plasmids
    Pduo Plasmids, supplied by InvivoGen, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pduo+plasmids/pDUO2-mCD14+TLR2/pmc11341917-140-12-16
    Average 91 stars, based on 7 article reviews
    pduo plasmids - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Growth phase matters: Boosting immunity via Lacticasebacillus‐derived membrane vesicles and their interactions with TLR2 pathways
    Article Snippet: For statistical analysis, ANOVA followed by Dunnett's multiple comparison test in GraphPad Prism 6 (GraphPad Software, San Diego, California, USA) was used; three independent experiments were performed. .. HEK293 (ATCC, USA) transfectants TLR2/CD14, TLR6/2 and TLR1/2 were stably transfected with pDUO plasmids purchased from InvivoGen (USA) according to the manufacturer's instructions. pDUO‐hCD14/TLR2, pDUO‐hTLR1/TLR2 pDUO‐hTLR6/TLR2 are expression vectors designed to co‐express CD14 and TLR2 genes, TLR1 and TLR2 genes and TLR6 and TLR2 genes. .. Further, commercially available HEK293 cells stably transfected with the human TLR4a, MD2 and CD14 genes, NOD1 genes and NOD2 genes were used (293/hTLR4A‐MD2‐CD14, 293‐hNOD1 and 293‐hNOD2 cells) and HEK 293 control cell line (293/null cells) was tested as a scrambled control (InvivoGen, USA).

    Transfection:

    Article Title: Growth phase matters: Boosting immunity via Lacticasebacillus‐derived membrane vesicles and their interactions with TLR2 pathways
    Article Snippet: For statistical analysis, ANOVA followed by Dunnett's multiple comparison test in GraphPad Prism 6 (GraphPad Software, San Diego, California, USA) was used; three independent experiments were performed. .. HEK293 (ATCC, USA) transfectants TLR2/CD14, TLR6/2 and TLR1/2 were stably transfected with pDUO plasmids purchased from InvivoGen (USA) according to the manufacturer's instructions. pDUO‐hCD14/TLR2, pDUO‐hTLR1/TLR2 pDUO‐hTLR6/TLR2 are expression vectors designed to co‐express CD14 and TLR2 genes, TLR1 and TLR2 genes and TLR6 and TLR2 genes. .. Further, commercially available HEK293 cells stably transfected with the human TLR4a, MD2 and CD14 genes, NOD1 genes and NOD2 genes were used (293/hTLR4A‐MD2‐CD14, 293‐hNOD1 and 293‐hNOD2 cells) and HEK 293 control cell line (293/null cells) was tested as a scrambled control (InvivoGen, USA).

    Expressing:

    Article Title: Growth phase matters: Boosting immunity via Lacticasebacillus‐derived membrane vesicles and their interactions with TLR2 pathways
    Article Snippet: For statistical analysis, ANOVA followed by Dunnett's multiple comparison test in GraphPad Prism 6 (GraphPad Software, San Diego, California, USA) was used; three independent experiments were performed. .. HEK293 (ATCC, USA) transfectants TLR2/CD14, TLR6/2 and TLR1/2 were stably transfected with pDUO plasmids purchased from InvivoGen (USA) according to the manufacturer's instructions. pDUO‐hCD14/TLR2, pDUO‐hTLR1/TLR2 pDUO‐hTLR6/TLR2 are expression vectors designed to co‐express CD14 and TLR2 genes, TLR1 and TLR2 genes and TLR6 and TLR2 genes. .. Further, commercially available HEK293 cells stably transfected with the human TLR4a, MD2 and CD14 genes, NOD1 genes and NOD2 genes were used (293/hTLR4A‐MD2‐CD14, 293‐hNOD1 and 293‐hNOD2 cells) and HEK 293 control cell line (293/null cells) was tested as a scrambled control (InvivoGen, USA).



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    Image Search Results


    Recombinant LTIIb-B5 protein expression, purification, and characterization ( A ) Left panel, purified LTIIb-B5 protein with Coomassie blue staining; right panel, Western blot. Pentameric LTIIb-B5 is located at ~50 kDa, monomeric LTIIb-B5 at ~12 kDa (denatured). ( B ) Pentameric and monomeric LTIIb-B5 formation under different biochemical conditions. Boiled proteins were heated to 100 °C for 5 min. ( C ) FPLC size-exclusion elution profile for LTIIb-B5 (eluted at 55 mL, corresponding to ~70 kDa). Four marker proteins are Ribonuclease (13.7k), Chymotrypsinogen (25k), Ovalbumin (43k) and Albumin (66.5k). Results indicate the presence of LTIIb-B5 pentamers in solution. ( D ) 1 H- 15 N HSQC spectrum of LTIIb-B5. Peak dispersion indicates dynamic folded structure. ( E ) TLR2/1 signal functional assay results for LTIIb-B5. HEK 293A cells contained over-expressed hTLR1/hTLR2 receptor and NF-kB-driven luciferase due to transient transfection. Transfected cells were treated with diluted LTIIb-B5 and Pam3CSK4 (1 pg/mL to 10 μg/mL) and held for 5 h at 37 °C. TLR2/1 activity was detected concurrently with luciferase activity.

    Journal: Vaccines

    Article Title: Type IIb Heat Labile Enterotoxin B Subunit as a Mucosal Adjuvant to Enhance Protective Immunity against H5N1 Avian Influenza Viruses

    doi: 10.3390/vaccines8040710

    Figure Lengend Snippet: Recombinant LTIIb-B5 protein expression, purification, and characterization ( A ) Left panel, purified LTIIb-B5 protein with Coomassie blue staining; right panel, Western blot. Pentameric LTIIb-B5 is located at ~50 kDa, monomeric LTIIb-B5 at ~12 kDa (denatured). ( B ) Pentameric and monomeric LTIIb-B5 formation under different biochemical conditions. Boiled proteins were heated to 100 °C for 5 min. ( C ) FPLC size-exclusion elution profile for LTIIb-B5 (eluted at 55 mL, corresponding to ~70 kDa). Four marker proteins are Ribonuclease (13.7k), Chymotrypsinogen (25k), Ovalbumin (43k) and Albumin (66.5k). Results indicate the presence of LTIIb-B5 pentamers in solution. ( D ) 1 H- 15 N HSQC spectrum of LTIIb-B5. Peak dispersion indicates dynamic folded structure. ( E ) TLR2/1 signal functional assay results for LTIIb-B5. HEK 293A cells contained over-expressed hTLR1/hTLR2 receptor and NF-kB-driven luciferase due to transient transfection. Transfected cells were treated with diluted LTIIb-B5 and Pam3CSK4 (1 pg/mL to 10 μg/mL) and held for 5 h at 37 °C. TLR2/1 activity was detected concurrently with luciferase activity.

    Article Snippet: HEK 293A cells were seeded and held overnight in culture dishes (6 × 10 6 cells/dish) prior to co-transfection with pDUO-hTLR1/hTLR2 plasmids (InvivoGen, San Diego, CA, USA) (7.5 μg/dish) and pGL4.32 (luc2p/NF-κB-RE/Hygro) vectors (Promega) (3 μg/dish) using Turbofect (Thermo Fisher Scientific, Waltham, IL, USA) transfection reagent.

    Techniques: Recombinant, Expressing, Purification, Staining, Western Blot, Marker, Dispersion, Functional Assay, Luciferase, Transfection, Activity Assay